货号
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货号
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描述
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个/盒
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价格
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60641
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μ-Proteofection Kit VI, for direct transport of proteins through the cell membrane, 15 μ-Slides VI 0.4, ibiTreat, sterile, 250 μl PROTEOfectene?, 100 μg/ml R-Phycoerythrin in 100 μl PBS
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1
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￥9810
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60642
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μ-Proteofection Kit VI AB, for direct transport of antibodies through the cell membrane, 15 μ-Slides VI 0.4, ibiTreat, sterile, 250 μl PROTEOfectene? AB, 100 μg/ml FITC-IgG in 100 μl PBS
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1
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￥9810
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For the direct insertion of proteins (e.g., antibodies) into the cytosol of living cells
Cell culture, proteofection, and microscopy – all in one slide
Reagents optimized for proteins or antibodies, respectively
Fast and simple protocol
Applications:
Protein localization through fluorescent antibodies
Intracellular competitive protein inhibition
Protein studies in knock down organisms
Technical Features:
90 proteofection experiments per kit
PROTEOfectene? optimized for protein insertion
PROTEOfectene? AB optimized for antibody insertion
The kit contains PROTEOfectene? (AB), appropriate positive control reagents, and μ-Slide VI 0.4
Specifications / Content:
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μ-Slide (ibidi)
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μ-Slide VI 0.4, ibiTreat (15x)
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Reagent (Biontex)
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PROTEOfectene? (250 μl)
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Positive control (Biontex)
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R-Phycoerythrin,
100 μg/ml in PBS (100 μl)
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μ-Slide (ibidi)
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μ-Slide VI 0.4, ibiTreat (15x)
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Reagent (Biontex)
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PROTEOfectene?AB (250 μl)
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Positive control (Biontex)
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FITC-IgG, 100 μg/ml
in PBS (100 μl)
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The PROTEOfectene? reagents (Biontex Laboratories GmbH) were developed to enable the direct uptake of proteins and antibodies through the lipid membrane of cells by endocytosis. Using this procedure, shuttled proteins reach the cytosol and act directly in signal transduction. If the antibodies/proteins are labeled, their fate can be followed directly using microscopy.
To ensure that the proteofection procedure is working, either a fluorescent protein (R-Phycoerythrin) or an antibody (FITC-IgG) is included in the kit. These positive controls are useful tools for an easy evaluation of the experimental results.
Imaging of HeLa cells after proteofection with FITC-IgG (positive control included in the μ-Proteofection Kit VI)